Twenty eight anaerobic bacteria were screened in the soil around Ebetsu city, and identified as Clostridium species by homology analysis of l6SrRNA. Of these, 2 bacteria (Clostridium acidisoli). No. 11 and No. 22, have a hydrogenase gene and produce hydrogen gas in the scallop digestive gland homogenates.

Hydrogen fermentation by these bacteria effectively decreased the protein content in the scallop digestive gland homogenates by 25%.

These results demonstrate that use of hydrogen-producing fermentation of soil Clostridium bacteria might decrease the solid waste produced in seafood processing