The sweet Japanese
cooking wine called mirin is traditionally made from glutinous rice. To develop
a mirin-Iike sweetener, high-starch produce (potatoes, edible lily bulbs,
pumpkins) produced in Hokkaido was examined as raw materials. The antioxidative
activities of these materials were also investigated. Koji (a preparation that
is obtained by growing Aspergillus oryzae on boiled rice, barley, soybeans or
the like and that is used as a starter for the fermentation in sake and
soy-sauce production), heat-treated peeled potato, shochu spirits containing 35%(
v/v) ethanol or 35% (w/v) aqueous ethanol, and commercially available powdered
amylase and protease enzyme were mixed in a weight ratio of 0.4: 2.0:1.0:0.005.
After incubation at 30fC for 45 or 60 days, the potato mirin was recovered. Lily
bulb and pumpkin mirins were prepared similarly.
1) The yield ratio and
glucose recovery ratio were slightly higher for enzyme-treated (0'15%) potato mirin
than for enzyme-untreated potato mirin; the total nitrogen recovery ratio was
greatly increased by enzyme addition.
2) The yield ratio of
the potato mirin (70.5%) as higher than those of the enzyme-treated pumpkin and
lily bulb mirins.
3) The total sugar
content (glucose) of the enzyme-treated potato mirin (25.2%) as about a half that
of commercially available rice mirin. The total amino acid content (1.4%) was
about 6 times higher than that of commercial rice mirin.
4) The antioxidative
activity of the enzyme-treated potato mirin, measured as butylated
hydroxyanisole (BHA) equivalent, was 109 mg / 100 ml. This was the highest
among the potato, lily and pumpkin mirins.