In order to improve the quality of raw Chinese noodles
produced in Hokkaido, we researched the microflora in the food. Bacteria were
isolated on an agar plate from the Chinese noodles after preservation, and
identified on the basis of their nucleotide sequences of the 16S ribosomal RNA
gene. Nine kinds of Chinese noodles produced by seven manufacturers in Hokkaido
and one manufacturer outside of Hokkaido were preserved at 25fC for seven days
and their microfloras were analyzed. As a result, these products were divided
into two types; one type in which a species closely related to A. viridans is most
predominant, and another type in which a kind of unknown gram-negative and rod
bacteria is most predominant. Such differences of microflora in the Chinese
noodles were inferred to be due to whether or not it contained vitamin B2,
saccharide or gardenia yellow as food-additives. When the representatives of
the former and later types of noodles were preserved at 25'C for four weeks,
predominant bacteria were a species closely related to A. viridans and alkaliphilic
Bacillus sp. in the former, and the above mentioned gram-negative and rod
bacteria and a species closely related to Salinicoccus roseus in the later.